Cell Viability Assay Kit (CQBL-05K)

This homogeneous assay involves simply adding a single reagent, the CellQuanti-Blue reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570 nm, emission wavelength = 590 - 620 nm) after an incubation step. The CellQuanti-Blue reagent, like other resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye resazurin which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The CellQuanti-Blue reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis. This product is for Research Use only and is not intended for diagnostic purposes.

• Homogeneous assay for cell viability, proliferation, cytotoxcity and HTS for anticancer agents.
• Method: Fluorometric assay (530/590nm). A spectrophotometer or plate reader is required, but not provided.
• Samples: Cell culture. Species: All. Detects 100 cells
• Procedure: 1-5 hrs. Size: 5000 tests per kit