D-Lactate Assay Kit (EDLC-100)

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems' EnzyChrom lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample. This product is for Research Use only and is not intended for diagnostic purposes.

• For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects.
• Method: Colorimetric assay (565 nm). A spectrophotometer or plate reader is required, but not provided.
• Samples: Serum, plasma, cell culture media etc. Species: All. Detects 0.05 mM
• Procedure: 20 min. Size: 100 tests per kit