IB48134 50 Piece Midi Flextube Kit with 1K MWCO, 800mL Volume

IBI Midi flextubes are typically used for the extraction of proteins, RNA, DNA, or oligonucleotides (greater than 20nt) from polyacrylamide, agarose, and or any other gel matrix in a running buffer. They are also used for dialysis or buffer change of small volumes (50-800 microliter), preparation of protein samples for MALD-MS, 2D gel dots extraction, and sample concentrations. IBI MIDI FlexTubes are tested by using them in extraction of proteins, RNA,or DNA fragments of various sizes from either agarose or polyacrylamide gels. IBI MIDI flextube kits are also tested for simple dialysis of salts or buffer exchanges. The quality of the isloated protein RNA, or DNA fragments, or of the sample after dialysis, is checked by several assays commonly used for proteins, nucleic acids, and dialysis. These devices combine two modes of action, electro-elution of macromolecules from polyacrylamide or agarose gels and buffer exchange of small volume samples. These devices allow rapid run times and high performance in either mode, and extract the macromolecules without contamination. They are ideal for purification of very small quantities of proteins (up to 0.5 microgram), for automated protein sequencing, peptide mapping, and amino acid analysis. Membrane cut-off: 1K MWCO. Tube volume capacity: 800 microliter. Minimum amount of protein at the start of extraction: 0.5 microgram. Maximum size of the gel slice that can be inserted into the tube: 1cm x 0.5cm. Volume of sample for dialysis: 50-800 microliter. Membrane: Ultra-clean, sulfur and heavy metal free. EDTA treated. Yield of molecule recovery: 90 percent DNA or RNA from agarose gel, 90 percent DNA or RNA from polyacrylamide gel, 70 percent Protein from SDS-PAGE.

• Kit comes complete with (50) Midi flextubes (1K MWCO), and operation manual
• Extraction of proteins, RNA, DNA or oligonucleotides (greater than 20 nt) from polyacrylamide, agarose or any gel matrix in any running buffer
• Extraction of protein-protein, DNA-protein or RNA-protein complexes
• Dialysis or buffer exchange of small volumes (50-800 microliter)
• Preparation of protein samples for MALD-MS, samples concentration